Fig. 6

Comparison of differential gene expression as assessed by mRNA-seq and quantitative PCR, respectively. Positive log2-fold change indicates upregulation in ΔXT/FT relative to wildtype, negative log2-fold change indicates downregulation in ΔXT/FT. Values above the table indicate two-tailed p-values computed in unpaired t tests between ΔXT/FT and WT qPCR results. Red: not significant, yellow: moderately significant, green: highly significant. Not available (“NA”) indicates absence of qPCR signal. We kept for further analyses only genes where both qPCR and mRNA-seq indicated up-regulation or down-regulation, respectively. Gene numbers refer to NibSet-1 genes. The plot is limited to the range [− 10, + 10]