Fig. 4

Genuine CFP signals were observed under microscope for the in/del-activated CFP cells enriched by the flowcytometer. HEK293T cells having FsCFP reporter with RRS target sequence and corresponding wildtype or mutant gRNA were sorted for positive CFP signals as decribed in Fig. 2a. The fluorescence of these cells was examined under microscope. Besides the expected GFP and RFP signals, prominent CFP signal was also detected in these cells. As a control, cells carring only the FsCFP reporter don’t exhibit detectable CFP signal. Scale bars indicate 50 μm