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Table 4 Read quality filtering on two sequencing platforms

From: Impact of sequencing depth and technology on de novo RNA-Seq assembly

Platform

Name

Adapter

Low Quality

N%

DNBseq™

ERR1831362

125 926

0.26%

1 424 492

2.96%

769 503

1.60%

ERR1831363

79 533

0.27%

871 008

2.92%

479 635

1.61%

ERR1831364

127 069

0.23%

1 476 496

2.69%

869 764

1.58%

ERR1831365

103 994

0.29%

1 051 568

2.92%

588 427

1.63%

ERR1831366

110 324

0.25%

1 060 462

2.43%

711 236

1.63%

ERR1831367

132 461

0.24%

1 581 470

2.87%

901 123

1.64%

ERR1831368

133 444

0.25%

1 588 843

2.98%

875 865

1.64%

ERR1831369

152 027

0.23%

1 825 708

2.79%

1 049 309

1.60%

ERR1831370

83 674

0.28%

898 776

3.02%

484 541

1.63%

HiSeq

SRR1261168

3 497

0.00%

30 319 019

22.47%

466 900

0.35%

SRR1261170

509

0.00%

33 381 937

45.79%

6 147 822

8.43%

SRR950078

1 313 463

1.31%

20 004 668

19.93%

1 307 643

1.30%

SRR950080

2 022 559

2.20%

18 689 016

20.36%

1 194 269

1.30%

SRR950084

4 443 842

3.55%

25 596 160

20.46%

1 675 649

1.34%

  1. The columns show the number and percentage of reads filtered out based on them containing adapter sequence, having too many low quality bases, and having too many ambiguous bases