Fig. 1

Global gene expression profiles in anti-CD3-treated human T cells by RNA-seq data. The transcriptome was obtained in CD3 T cells collected from a healthy donor at two different moments, treated or untreated with anti-CD3 antibodies. Only genes with padj ≤ 0.05 were considered differentially expressed. a MA-plot with the global gene expression profile; red dots indicate up- or downregulated genes. b Venn diagrams showing an overlap of regulated expressed genes compared to the control, among different anti-CD3 treatments. c Clustering analysis and heatmap of gene expression based on fold change data. Cluster analysis was performed with 860 commonly regulated genes (shown in rows) for each sample (columns). Gradient colors from purple to gold represent lower to higher expression (range from − 9.27 to 9.22)