Fig. 4From: Cold seawater induces early sexual developmental stages in the BPG axis of European eel malesPituitary Lhβ histochemical identification. Confocal images of the immunofluorescence labelled European eel (Anguilla anguilla) male pituitaries, which showed the strongest Lhβ signal from each of the analyzed groups: the 2 week 10 °C pretreated group (T10; panel d, e, and f), the 2 week 20 °C pretreated group (T20; panel g, h, and i), and Control (Panel a, b, and c). “DAPI” indicates pictures filtered to only reveal fluorescents labeled to 4,6-diamidino-2-phenylindole dihydrochloride (Panel a, d, and g). “Lhβ” indicates pictures filtered to only reveal fluorescents labeled to luteinizing hormone beta subunit protein (Panel b, e, and h). “DAPI / Lhβ” indicates pictures filtered to reveal both fluorescents labelled to luteinizing hormone beta subunit protein and 4,6-diamidino-2-phenylindole dihydrochloride (Panel c, f, and i)Back to article page