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Fig. 4 | BMC Genomics

Fig. 4

From: Cold seawater induces early sexual developmental stages in the BPG axis of European eel males

Fig. 4

Pituitary Lhβ histochemical identification. Confocal images of the immunofluorescence labelled European eel (Anguilla anguilla) male pituitaries, which showed the strongest Lhβ signal from each of the analyzed groups: the 2 week 10 °C pretreated group (T10; panel d, e, and f), the 2 week 20 °C pretreated group (T20; panel g, h, and i), and Control (Panel a, b, and c). “DAPI” indicates pictures filtered to only reveal fluorescents labeled to 4,6-diamidino-2-phenylindole dihydrochloride (Panel a, d, and g). “Lhβ” indicates pictures filtered to only reveal fluorescents labeled to luteinizing hormone beta subunit protein (Panel b, e, and h). “DAPI / Lhβ” indicates pictures filtered to reveal both fluorescents labelled to luteinizing hormone beta subunit protein and 4,6-diamidino-2-phenylindole dihydrochloride (Panel c, f, and i)

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