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Fig. 1 | BMC Genomics

Fig. 1

From: Single-cell RNA-seq of esophageal squamous cell carcinoma cell line with fractionated irradiation reveals radioresistant gene expression patterns

Fig. 1

KYSE-180 cells acquired radioresistance in vitro. a. Morphological image of KYSE-180, KYSE-180-12 Gy and KYSE-180-30 Gy. b. Wound healing assay showed migration of KYSE-180, KYSE-180-12 Gy and KYSE-180-30 Gy. D0, Day 0; D4: Day 4. c. FACS analysis with Annexin V-PE and 7-AAD showing apoptosis results of KYSE-180, KYSE-180-12 Gy and KYSE-180-30 Gy. d. Transwell assay showed invasion of KYSE-180, KYSE-180-12 Gy and KYSE-180-30 Gy. e. Immunofluorescence analysis of γ-H2AX expression 2 h after 4 Gy of FIR in control KYSE-180 cells and in FIR-treated KYSE-180-12 Gy and KYSE-180-30 Gy cells. Green: γ-H2AX; Blue: DAPI. f. Surviving KYSE-180 cells with and without FIR exposure identified by CCK-8 assay. * means P < 0.05; ** represents P < 0.01

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