Fig. 4
Genome-wide association study of Met and Tau with 427 individuals. a, b The Manhattan and QQ plots of Met and Tau content. The negative log10-transformed P-values from the genome-wide scan are plotted against position in each of the 10 chromosomes. Red horizontal dashed line indicates the genome-wide significance threshold. c, f The 0.1-Mb region on each side of the peak SNP in scaffold618 (c) and scaffold801 (f), the position of peak SNP is indicated by a vertical red line with red triangle. The bottom panel shows the annotated genes of the 100-kb region and the LD analysis of pairwise SNP loci in the 100 kb-region on both sides of the leading SNP obtained by GWAS (P < 2 × 10− 4). d The candidate genes for Met content with one non-synonymous mutation in the coding region. e The left panel indicates the Met content in different genotypes of COOA gene. The right panel indicates the mRNA expression of COOA genes in high and low Met content oysters. Different letters indicate the significant differences (P < 0.05) and the error bars represent ± SD (n = 15). g The candidate genes for Tau content and different haplotypes with five significant SNP loci. h The left panel indicates the Tau content in different haplotypes of PLC genes. The right panel indicates the mRNA expressions of PLC genes in high and low Tau content oysters. Different letters indicate significant differences (P < 0.05) and error bars represent ± SDs (n = 15)