Fig. 4

Overexpression of Gh_A07G0747 (GhTOM1) causes hypersensitivity to ABA-elicited seed germination, root growth inhibition and fresh biomass accumulation in transgenic plants and WT. a Seed germination rate of transgenic lines and wild type on ABA 0.5 MS with (0, 0.5, 1 and 2 μM) for 10 days. b Quantitative determination of root lengths. c Quantitative comparisons of germination rate of the transgenic and wild type on ABA 0.5 MS with (0, 0.5, 1 and 2 μM) after 10 days. d Root elongation comparisons on 0.5 MS with varied ABA concentrations (0, 0.5, 1 and 2 μM). e Quantitative determination of fresh weight biomass of wild-type and both overexpressed lines. The seedlings were scored and photographed after 6 days post germination. Each experiment was repeated three times. Bar indicates standard error (SE). Different letters indicate significant differences between wild-type and OE lines (ANOVA; p < 0.05). CK: normal conditions