Fig. 4
Gene expression changes after pyramidotomy in neonates and adults. a, b, Volcano plots of P7 pyramidotomy vs P7 sham (a) or 8 W pyramidotomy vs 8 W sham groups (b). Vertical axes represent the −log10 adjusted P value, whereas the horizontal axes represent the log2 fold-change in expression levels in the P7 pyramidotomy group compared to those in the P7 sham group (a) or in the 8 W pyramidotomy group compared to those in the 8 W sham group (b). Broken lines indicate the level of 2-fold change. Points beyond the range are plotted on the edge of the graph in triangles. Red indicates adjusted P < 0.05 (Wald test). Because some genes were judged as outliers and omitted from some comparisons, the total number of genes was different. c, d, KEGG pathways significantly upregulated in P7 pyramidotomy group compared to that in the P7 sham groups (c) or in the 8 W pyramidotomy group compared to that in the 8 W sham (d) group are shown. Top 10 pathways among 30 (c) or 44 (d) total pathways for which the adjusted P was < 0.05 (two-sample t-test) are shown, and the complete lists are presented in Additional files 5 and 6