Fig. 8

Expression levels (FPKM) of genes encoding SNF1-related protein kinase-1 (SnRK1) type a, b and b*, in the embryo and endosperm of developing grain from wheat (Triticum aestivum) genotypes Spark and SR3. The plants were grown with sulphur either supplied (+) or withheld (−). Results for each homeologue are shown separately, as indicated. a Embryo, 14 dpa. b Endosperm, 14 dpa. c Embryo, 21 dpa. d Endosperm, 21 dpa. Gene reference numbers are given in Additional file 2. The increase in expression of the SnRK1b homeologues on chromosomes 3B and 3DL in response to sulphur deficiency in Spark endosperm at 21 dpa was significant (p < 0.01 and p < 0.001, respectively), as was the increase in expression of the 3B homeologue in the embryo (p < 0.01). The increase in expression of the 4BL and 4DL homeologues of the SnRK1b* gene in response to sulphur deficiency at 21 dpa was significant in SR3 embryo (p = 0.0426 and p = 0.0377, respectively), as was the increase in expression of the 4DL homeologue in Spark endosperm (p = 0.0404)