Fig. 1From: RNAi based transcriptome suggests genes potentially regulated by HSF1 in the Pacific oyster Crassostrea gigas under thermal stressEffects of HSF1 gene silencing on HSF1 expression in C. gigas gills. The control represents group 1, RNAi&HS represents group 4 (heat shock following RNA interference), RNAi represents group 3 (RNA interference) a HSF1 mRNA expression detected by qRT-PCR (n = 15). b HSF1 protein content detected by western blotting (n = 2). Each band represents the protein content of an individual, with the upper band representing the content of the HSF1 protein and the lower band representing the content of β-tubulin. c Statistical analysis of HSF1 protein content detected by western blotting (n = 2). The error bar denotes the standard error of the mean; **, p < 0.05; *** p < 0.001Back to article page