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Fig. 1 | BMC Genomics

Fig. 1

From: Transcriptome profile of Corynebacterium pseudotuberculosis in response to iron limitation

Fig. 1

C. pseudotuberculosis growth assays. Growth proliferation was determined by measuring the OD of low iron (LI) and high iron (HI) cultures at 600 nm of the wild-type (parent) T1 strain and the Cp13 mutant strain, reflecting the growth dynamics of the bacterial strains in the culture media over the different time points. BHI broth was supplemented with 250 μM of the iron chelator 2,2′-dipyridyl (DIP) to iron stress the bacteria. The stress was confirmed at 6h30min with a significant reduction in the final culture density of both strains. At the 6h30min, both strains presented a final LI culture density of 63 and 52.6% of the bacteria grown in the high iron BHI broth a wild-type T1 strain and b Cp13 mutant. C. pseudotuberculosis viability was measured by determining the number of CFU/mL after 6h30min of incubation in a low iron (LI) dipyridyl-chelated and high iron (HI) non-chelated media. Average log10 (CFU/mL) along with the standard deviation (error bars) for the experimental cultured conditions (HI vs LI) from three individual biological replicates are represented. Differences between HI and LI for the wild-type T1 strain and Cp13 mutant were shown to be statistically significant using a two-tailed t test with a p-value < 0.001(****). Log10 (CFU/mL) in the c wild-type T1 strain and the d Cp13 mutant

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