Table 1 Summary of virus assemblies, Senegal Anopheles sample pools
Reference virus | 3NCBI classification reference virus | Closest relative (BLASTX) | Assembled sequence | Alignment length (identities, positives, gaps, frame) | Score (E-value) Blastx | Alignment coordinates (start..end Assembled sequence-start..end Closest relative) |
|---|---|---|---|---|---|---|
DsRNA virus environmental sample clone mill.culi_contig84 | Viruses; dsRNA viruses; environmental samples. | gi|766989332|gb|AJT39580.1| proline-alanine-rich protein [dsRNA virus environmental sample] (975 aa) | PrAlaRichProt_EnvVirDak (1345 nt) | 308 (71,122,6,+ 1) | 77(1e-11) | 4..909–653..954 |
Homalodisca vitripennis reovirus segment S3 | Viruses; dsRNA viruses; Reoviridae; Sedoreovirinae; Phytoreovirus; unclassified Phytoreovirus | gi|226423326|ref.|YP_002790886.1| major core protein [Homalodisca vitripennis reovirus] (1021 aa) | CP_HVreovirusDak (5674 nt) | 483 (113,206,21,+ 3) | 90(2e-14) | 3774..5159–211..671 |
Daeseongdong virus 1 strain A12.2708/ROK/2012 | Viruses; unclassified viruses. | gi|959121745|ref.|YP_009182191.1| putative RNA-dependent RNA polymerase [Daeseongdong virus 1]. (2361 aa) | RdRP_DaeseondongVirDak (2530 nt) | 503 (125,225,6,-1) | 135(1e-28) | 875..2365–45..489 |
Ixodes scapularis associated virus 2 isolate A1, partial genome | Viruses; unclassified viruses. | gi|669132782|gb|AII01812.1| hypothetical protein, partial [Ixodes scapularis associated virus 2] (337 aa) | HP1.1_IxodesVirDak (2820 nt) | 340 (141,210,2,-2) | 267(3e-77) | 105..1118–3..337 |
HP1.2_IxodesVirDak (2561 nt) | 319 (116,157,11,-1) | 159(4e-39) | 156..1079–3..319 | |||
Uncultured virus isolate acc_7.4 | Viruses; environmental samples. | gi|545716017|gb|AGW51759.1| RNA-dependent RNA polymerase-like protein [uncultured virus] (473 aa) | RdRP_UncVir1Dak (1488 nt) | 475 (217,295,8,-2) | 377(e-121) | 57..1457–1..471 |
Uncultured virus isolate acc_1.3 | Viruses; environmental samples. | gi|545716010|gb|AGW51755.1| RNA-dependent RNA polymerase-like protein, partial [uncultured virus] (1445 aa) | RdRP_UncVir2Dak (2011 nt) | 236 (168,200,0,+ 1) 178 (146,157,0,+ 3) 91 (71,80,0,+ 2) | 353 (0.0) 298 (0.0) 152 (0.0) | 25..732–58..293 1020..1553–408..585 752..1024–320..410 |
American dog tick phlebovirus isolate FI3 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; Bunyavirales; Phlebovirus; unclassified Phlebovirus. | gi|734669629|gb|AJA31764.1| nucleocapsid, partial [American dog tick phlebovirus] (245 aa) | NuclCap1.1_ADTphlebovirusDak (1105 nt) | 142 (55,75,11,+ 2) | 75(5e-12) | 191..583–38..175 |
NuclCap1.2_ADTphlebovirusDak (1148 nt) | 151 (53,71,9,+ 2) | 72(3e-11) | 173..598–27..175 | |||
Culex tritaeniorhynchus rhabdovirus RNA, complete genome, strain: TY | Viruses; ssRNA viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae. | gi|700895640|ref.|YP_009094323.1| large protein [Culex tritaeniorhynchus rhabdovirus] (2123 aa) | LP_CulexRhabdovDak (1526 nt) | 266 (101,149,0,+ 3) 108 (33,45,0,+ 2) 72 (50,59,0,+ 2) | 177(6e-52) 56(6e-52) 115(2e-23) | 498..1295–155..417 149..472–38..145 1307..1522–593..664 |
Phasi Charoen-like virus | Viruses; ssRNA viruses; ssRNA negative-strand viruses; Bunyaviridae; unclassified Bunyaviridae. | gi|664682120|gb|AIF71032.1| nucleocapsid [Phasi Charoen-like virus] (268 aa) | NuclCap1.2_PCLVDak (1104 nt) | 82 (49,57,1,+ 3) | 84(6e-26) | 699..941–116..197 |
NuclCap1.3_PCLVDak (1125 nt) | 97 (57,74,0,+ 2) | 115(7e-48) | 119..409–65..161 | |||
NuclCap1.4_PCLVDak (1144 nt) | 262 (114,167,3,+ 3) | 217(7e-64) | 153..929–6..267 | |||
gi|870898373|gb|AKP18600.1| glycoprotein [Phasi Charoen-like virus] (1237 aa) | GP_PCLVDak (3887 nt) | 1185 (436,711,15,+ 1) | 894 (0.0) | 91..3600–20..1197 | ||
gi|664682116|gb|AIF71030.1| RNA-dependent RNA polymerase [Phasi Charoen-like virus] (2217 aa) | RdRP_PCLVDak (6711 nt) | 2213 (1042,1432,52,+ 1) | 1944 (0.0) | 148..6630–8..2196 | ||
Wellfleet Bay virus isolate 10–280-G segment 4 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; Orthomyxoviridae; Quaranjavirus; unclassified Quaranjavirus. | gi|727361119|ref.|YP_009110683.1| nucleoprotein [Wellfleet Bay virus] (537 aa) | Nuclprot1.1_WBvirDak (1973 nt) | 213 (60,101,7,+ 3) | 68(2e-08) | 705..1322–149..354 |
Nuclprot1.2_WBvirDak (3252 nt) | 459 (114,197,39,+ 3) | 117(8e-24) | 1413..2672–11..451 | |||
Wuhan Mosquito Virus 9 strain JX1–13 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; unclassified ssRNA negative-strand viruses. | gi|752455731|gb|AJG39214.1| ORF1 [Wuhan Mosquito Virus 9] (279 aa) | ORF1_Wuhan9virDak (1574 nt) | 204 (57,94,2,+ 3) | 86(3e-15) | 867..1472–13..210 |
Wuhan Mosquito Virus 1 strain WT3–15 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; unclassified ssRNA negative-strand viruses. | gi|752455880|gb|AJG39296.1| glycoprotein precursor [Wuhan Mosquito Virus 1]. (681 aa) | GP_Wuhan1virDak (3547 nt) | 153 (97,122,11,+ 1) | 219(3e-56) | 2023..2448–243..395 |
Wuhan Spider Virus strain SYZZ-2 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; unclassified ssRNA negative-strand viruses. | gi|752455826|gb|AJG39269.1| RNA-dependent RNA polymerase [Wuhan Spider Virus] (2251 aa) | RdRP1.1_WSVDak (998 nt) | 354 (87,154,26,+ 3) | 104(1e-20) | 6..989–1239..1589 |
RdRP1.2_WSVDak (2083 nt) | 730 (209,327,36 + 2) | 263(1e-70) | 2..2083–531..1243 | |||
RdRP1.3_WSVDak (1070 nt) | 377 (108,172,23 + 2) | 139(9e-32) | 2..1063–579..951 | |||
Xincheng Mosquito Virus strain XC1–6 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; unclassified ssRNA negative-strand viruses. | gi|752455743|gb|AJG39224.1| ORF1 [Xincheng Mosquito Virus] (462 aa) | ORF1_XinchengVirDak (947 nt) | 299 (73,122,11,+ 2) | 55(5e-05) | 81..944–22..311 |
gi|752455744|gb|AJG39225.1| ORF2 [Xincheng Mosquito Virus] (437 aa) | ORF2_XinchengVirDak (1726 nt) | 165 (44,88,2,+ 1) | 67(2e-08) | 145..633–260..422 | ||
gi|752455745|gb|AJG39226.1| glycoprotein [Xincheng Mosquito Virus] (648 aa) | GP_XinchengVirDak (5993 nt) | 592 (352,445,0,+ 3) | 751 (0.0) | 3720..5495–16..607 | ||
gi|752455746|gb|AJG39227.1| RNA-dependent RNA polymerase [Xincheng Mosquito Virus] (2026 aa) | RdRP1.1_XinchengVirDak (11707 nt) | 2001 (733,1101,50,+ 3) | 1226 (0.0) | 5571..11423–28..1977 | ||
RdRP1.2_XinchengVirDak (11722 nt) | 2001 (733,1101,50,+ 3) | 1226 (0.0) | 5586..11438–28..1977 | |||
RdRP1.3_XinchengVirDak (11710 nt) | 2001 (734,1104,50,+ 3) | 1229 (0.0) | 293..6145–28..1977 | |||
RdRP1.4_XinchengVirDak (11694 nt) | 2001 (734,1104,50,-3) | 1229 (0.0) | 293..6145–28..1977 | |||
RdRP1.5_XinchengVirDak (11728 nt) | 1955 (733,1093,47,-1) | 1233 (0.0) | 440..6163–28..1924 | |||
RdRP1.6_XinchengVirDak (11716 nt) | 1955 (733,1093,47,+ 2) | 1233 (0.0) | 5561..11284–28..1924 | |||
RdRP1.7_XinchengVirDak (6128 nt) | 1962 (1088,1397,9,-3) | 2179 (0.0) | 124..5982–36..1994 | |||
Xinzhou Mosquito Virus strain XC3–5 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; unclassified ssRNA negative-strand viruses. | gi|752455830|gb|AJG39271.1| RNA-dependent RNA polymerase [Xinzhou Mosquito Virus]. (2022 aa) | RdRP1.1_XinzhouVirDak (7527 nt) | 2017 (1403,1670,3,+ 1) | 2863 (0.0) | 1315..7356–1..2013 |
RdRP1.2_XinzhouVirDak (7524 nt) | 2020 (1403,1676,6,+ 1) | 2864 (0.0) | 1312..7353–1..2013 | |||
Sunn-hemp mosaic virus | Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Virgaviridae; Tobamovirus. | gi|12643499|sp.|P89202.2|RDRP_SHMV RecName: Full = Replicase large subunit] (1629 aa) | RdRP_SHMVDak (1216 nt) | 365 (105,171,13,-1) | 125(4e-27) | 2..1057–932..1240 |
Omono River virus | Viruses; dsRNA viruses | gi|307933351|dbj|BAJ21511.1| RNA-dependent RNA polymerase [Omono River virus] (621 aa) | RdRP_OmonoVirDak (613 nt) | 194 (71,110,1,-1) | 131(1e-31) | 5..583–135..323 |
Beaumont virus strain 6 | Viruses; ssRNA viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae. | gi|550631504|gb|AGX86091.1| RNA-dependent RNA polymerase, partial [Beaumont virus] (1568 aa) | RdRP_BeaumontVirDak (805 nt) | 153 (76,98,0,+ 3) | 142(9e-34) | 3..461–417..569 |