Fig. 2

The mmu-miR-190a-3p involved module in the hippocampal tissue. (a) Clustering dendrograms of genes according to the topological overlap to distinguish the subgroup and used same color to assign highly correlated modules. As the result, 25 co-expression modules were constructed and painted with different colors. Pearson correlation analysis was used to determine whether co-expression modules are correlated with mmu-miR-190a-3p. Red color indicates the module was positively correlated with mmu-miR-190a-3p, and green demonstrates negative correlation. (b) Overview of candidate genes modified by the mmu-miR-190a-3p. Nodes are defined as the target genes modulated by the mmu-miR-190a-3p which was representing by red ellipse. The 4 pink rectangular represented the prediction results from miRwalk3.0 databases. (c) Distribution of Pearson correlation coefficients of predicted mmu-miR-190a-30-mRNA pairs. We determined the correlation between the expression of that miRNA and the expression of a predicted mRNA targets in all samples by calculating a Pearson correlation coefficient. This process was repeated for all targets and the red line indicated the distribution of these coefficients as a density plot. The blue line as the control curve represented the distribution of the coefficient of others unrelated mRNA with mmu-miR-190a-3p. A lift shift of the red line comparing with the red line demonstrated that the potential targets for mmu-miR-190a-3p are preferentially more negative correlated with targets gene than random