Table 2 List of primer sequences of randomly selected unigenes used for qRT-PCR analysis
Gene ID | Tm | Primer F (5′-3′) | Primer R (5′-3′) |
|---|---|---|---|
c99496_g1 | 56.5 | GAAAAACCCAGACTCAATCCCA | ACCAAGCTTCTCAATCACAACG |
c71418_g1 | 58.5 | ACCTCGGAGAAGGGTGGAC | AACAAGGATTGGGGGGAAA |
c96971_g1 | 53.0 | TACTACTTCCGCATCACCAAC | GGACTTCAACGACCACCATAT |
c81457_g1 | 56.0 | TTTGCTGAGATTATTATTTGTGGG | AAGATTAGGGTAAGGTTAGCGTGT |
c100796_g1 | 60.5 | GGTTTGTTGAAGCAGAAGGCG | GGTGTTAGGGAAGGTGAGGGC |
c101069_g2 | 57.3 | AGAGAGAAAGCGATAATGGAAGG | GTTGAAGGCGAGCGTAGGTG |
c85087_g1 | 52.5 | CAATTACAATTCACATTCAACAA | ATAGCATCCCTACCATTACAAAC |
c77820_g1 | 53.0 | AGAAAGAAATCCCTAAAGGGG | AGGAATGCTGTGTAGCACAAC |
c101781_g1 | 53.0 | AGAGAATAATGCAGGAGCACG | GCTGAGACAAGTTGGAAGAGTG |
c89063_g2 | 57.8 | TGCTACTTCCCCCACTGCTG | ACACTCCCCTTGTTCCACCC |
c87939_g1 | 55.0 | GGGAAAAAGACTGAAGAGAAAATAG | GAGAAAGGTAAGCTGAAACAAAATC |
c98652_g1 | 57.5 | GCAGTAGTGGGACGAGACAGG | GAGGGGAGTAGATGGGTGGAG |