Fig. 5

Minor intron splicing can be efficient. a Table showing the number of retained minor introns and flanking major introns across eleven mouse tissues. Above are schematics exemplifying the 6 different categories. b Heatmap of MSI values for minor introns, upstream major introns, and downstream major introns across eleven mouse tissues. c Bargraphs showing retention levels for the minor intron, upstream and downstream major introns of Pcyt2 and Tcp1 in different tissues as determined by RNAseq. Data are represented as mean ± SEM. Above is the gene schematic with intron sizes. Minor intron is depicted by the red line. MSI = mis-splicing index; E = exon; pctl = percentile. Significance was determined by one-way ANOVA, followed by post-hoc Tukey test. * = P < 0.05; ** = P < 0.01; n.s. = not significant