Fig. 1

Characteristics of transcript isoforms in N. lugens detected by PacBio sequencing. a, Distribution of insert reads’ length in three SMRT cells. b, Comparison of number of isoforms per gene between transcripts in the reference genome NilLug1.0 and PacBio sequencing dataset. c, Correlation between the number of isoforms per gene and number of exons per gene in the PacBio sequencing data (lower left graph), density profile of the number of exons per gene (right graph) and density distribution of the number of isoforms per gene (upper left graph). d, Correlation between the number of isoforms per gene and gene expression level according to PacBio sequencing results. e, Comparison of length of transcripts in the reference genome NilLug1.0 and PacBio sequencing isoforms (with significance of difference determined by Wilcoxon rank sum test). f, Percentages of unmapped and filtered PacBio reads mapped to the genomes of 15 species using Blastx with an E < 1e-5