Fig. 5

Validation of HeliScopeCAGE expression for six differentially expressed transcription factors with qRT-PCR. Quantitative real-time PCR validation for the expression of Atf4, Insm1, Mxi1, Pcbp1, Rfx3 and Scrt2. Gene expression is measured – relative quantity (RQ, quantity relative to H2O negative control) for qRT-PCR and tpm for CAGE genes were shown on y-axis. Expression at E13, E15 and E18 (x-axis) were sampled for prediction and validation groups to representative embryonic developmental stages. Expression pattern is used as indication for the accuracy of CAGE data