Fig. 8

Expression of Atf4, Rfx3 and Scrt2 in the developing cerebellum demonstrated by in-situ hybridization. (Top row) At E13.5, all three genes are prominently expressed in the neuroepithelial ventricular zone (VZ). In addition, Atf4 and Rfx3 expression is observed in the subpial stream (SS) and nuclear transitory zone (NTZ). Rfx3 is also expressed in the rhombic lip (RL). (Middle row) At E15.5, expression of all three genes remain prominent at the VZ. Expression of all three genes is now seen also in the external geminal layer (EGL) that contain the granule cell precursors. (Bottom row) At E18.5, expression in the VZ has ceased for Atf4 and Scrt2, while Rfx3 expression is still observed in the VZ. All three genes are prominently expressed in the EGL at this age