Table 1 Metabolites identified in bull spermatozoa by GC-MS
From: Uncovering sperm metabolome to discover biomarkers for bull fertility
Compounds | RT | TI | QI 1 | QI 2 | Mean relative abundance (± SD) | Min/Max values | FC | t-test P-value |
|---|---|---|---|---|---|---|---|---|
Amino acids, peptides, and analogs | ||||||||
 GABA | 11.40 | 147 | 73 | 174 | 7.7E-03 ± 8.2E-04 | 3.6E-03/0.01 | 1.61 | 0.023 |
 L-Serine | 13.19 | 147 | 73 | 103 | 6.1E-04 ± 1E-04 | 4.6E-05/1E-03 | 1.33 | 0.211 |
Carbohydrates and carbohydrate conjugates | ||||||||
 Glycerol | 12.62 | 147.2 | 73 | 205.2 | 0.09 ± 4.7E-03 | 0.075/0.123 | 1.04 | 0.390 |
Carboxylic acids and derivatives | ||||||||
 Acetic acid | 13.29 | 191 | 73 | 146.8 | 8.2E-04 ± 1E-04 | 1.2E-04/0.002 | 0.68 | 0.211 |
 Acetate | 10.10 | 147 | 73 | 155 | 6.0E-03 ± 5.9E-03 | 3.1E-03/9.1E-03 | 0.81 | 0.142 |
Fatty acids and conjugates | ||||||||
 Nonanoic acid (C9:0) | 13.94 | 117 | 73 | 202 | 4.5E-03 ± 4E-04 | 2E-03/6 E-03 | 1.01 | 0.246 |
 Azelaic acid (C9H16O4) | 20.51 | 317.2 | 73 | 201 | 0.02 ± 2.4E-04 | 1E-03/ 3E-03 | 1.09 | 0.397 |
 Oleic acid (C18:1 n-9) | 27.56 | 131.2 | 73 | 144.2 | 0.33 ± 0.02 | 0.256/0.446 | 0.99 | 0.481 |
 Oleanitrile (C18H33N) | 23.96 | 122.2 | 69.2 | 55 | 5.9E-03 ± 5.5E-04 | 0.018/0.035 | 1.05 | 0.395 |
 Palmitic acid (C16:0) | 23.54 | 313.4 | 73 | 117 | 5.9E-03 ± 5.5E-04 | 3E-03/ 8E-03 | 0.77 | 0.040 |
Inorganic compounds | ||||||||
 Phosphoric acid | 12.58 | 299 | 73 | 357 | 0.2 ± 0.06 | 0.065/0.82 | 0.73 | 0.228 |
 Borate | 6.59 | 221 | 73 | 157 | 0.071 ± 9.3E-03 | 0.033/0.12 | 0.91 | 0.366 |
 Phosphine | 9.53 | 116 | 73 | 204 | 0.14 ± 8.1E-03 | 0.109/0.176 | 0.93 | 0.390 |
Keto acids and derivatives | ||||||||
 2-Ketobutyric acid | 14.31 | 202.2 | 73 | 111.8 | 4.5E-04 ± 1E-04 | 1.1E-04/1 E-03 | 0.89 | 0.474 |
Organic acids and derivatives | ||||||||
 Lactic acid | 8.20 | 117 | 73 | 147 | 9.7E-04 ± 1E-03 | 3.4E-03/0.016 | 1.59 | 0.040 |
 Oxalic acid | 8.16 | 147 | 73 | 133 | 0.01 ± 4.3E-04 | 9.1E-03/0.013 | 1.02 | 0.403 |
 Urea | 11.83 | 147 | 73 | 189 | 5.9E-03 ± 8.2E-04 | 1.1E-03/9.1E-03 | 1.03 | 0.452 |
 Benzoic acid | 11.90 | 105 | 77 | 179 | 1.2E-03 ± 8.6E-05 | 9.9E-03/1.9E-03 | 1.46 | 0.034 |
 Carbonate | 12.32 | 191 | 73 | 199 | 5.7E-04 ± 1.4E-04 | 3.5E-07/0.001 | 1.00 | 0.493 |
 Carbamate | 7.651 8.019 | 147 174 | 73 73 | 204 198 | 0.05 ± 0.01 0.14 ± 1E-03 | 0.044/0.057 0.061/0.186 | 1.08 1.47 | 0.171 0.033 |
Steroids and steroid derivatives | ||||||||
 Cholesterol | 34.50 | 129.2 | 73 | 207.2 | 7.1E-03 ± 9.4E-04 | 3.7E-03/1.3E-02 | 1.03 | 0.442 |