Fig. 3
Parasite numbers in the brain in the in vivo experiment. Parasite numbers in the brain tissue from the T. gondii-infected wild-type (WT) and CCR5-deficient (KO) mice were determined by real-time quantitative PCR on the T. gondii B1 gene. a, 30 dpi; b, 7 dpi. Parasite numbers were compared using the student’s unpaired t-test between the WT and CCR5KO mice (*p > 0.05). Ct values higher than those for the uninfected samples were considered indicative of nonspecific amplification and were excluded from the plot. Each symbol represents the data point for one mouse, and the bars represent the mean value for all the group data points (uninfected WT, n = 3; uninfected CCR5KO, n = 3; infected WT, n = 5; infected CCR5KO, n = 7)