Fig. 1From: Molecular cloning and characterization of a grapevine (Vitis vinifera L.) serotonin N-acetyltransferase (VvSNAT2) gene involved in plant defenseN-acetyltryptamine production in Escherichia coli. VvGNAT genes and empty vector were transformed into E. coli. The expression of VvGNAT proteins was induced by the addition of IPTG, and enzyme activity was measured in the presence of 1 mM tryptamine for 12 h. The bacterial pellets were collected and used for the quantification of N-acetyltryptamine using HPLC. Data represent mean ± standard deviation (SD) of triplicate experimentsBack to article page