Skip to main content
Fig. 4 | BMC Genomics

Fig. 4

From: Molecular cloning and characterization of a grapevine (Vitis vinifera L.) serotonin N-acetyltransferase (VvSNAT2) gene involved in plant defense

Fig. 4

Sublocalization of VvSNAT2. a Transient expression of VvSNAT2-GFP in Arabidopsis protoplasts. pBI221-GFP/VvSNAT2 and control pBI221-GFP plasmids were introduced into Arabidopsis protoplasts via PEG-meditated transformation. The transformed protoplasts were incubated in the dark for 14 h and then visualized using confocal microscopy. b Immunogold labeling of VvSNAT2 using ultrathin sections of grapevine leaves. Sections were incubated with antiserum diluted 100-fold in 1% (w/v) BSA in TBS buffer for 1 h. After washing the samples with TBS buffer, gold-labeled sections were examined under a transmission electron microscope. Rabbit pre-immune serum was used as a control

Back to article page