Fig. 7

Phylogenomic relationship of three Gymnosporangium spp. with selected species in the Pucciniomycotina and identification of conserved proteins based on Multiclustering (MCL) analysis. A Maximum likelihood tree was constructed with RAxML using concatenated protein sequences from 1042 conserved single-copy genes found in the predicted proteomes of Gymnosporangium spp. teliospore transcriptomes and of four fungi selected in the Pucciniomycotina (Puccinia graminis f. sp. tritici, Melampsora larici-populina, Cronartium quercuum f. sp. fusiforme and Microbotryum lychnidis-dioicae). Bootstraps values are shown on branches (1000 replicates). Bold numbers shown at the species, genus or order levels, indicate lineage-specific clusters identified by the MCL analysis between the proteomes from the selected species, and bold numbers in bracket indicate the number of clusters remaining as specific after similarity search in the NCBI non-redundant protein database (Blastp; e-value ≤1e-5). The total number of proteins found in corresponding MCL clusters at each taxonomical level are shown non-bold below the cluster numbers