Table 6 Primers used in the qRT-PCR experiment
Primer name | Sequence | Efficiency (%) | R2 |
|---|---|---|---|
ALF1-F | 5′-CTG GTG ACG GAA GAA GAA GC-3′ | 98.13 | 0.9975 |
ALF1-R | 5′–CTT AAC CAG GCC ATT CCT CA–3’ |  |  |
Spz-F | 5′-CGA CGGA ATA CCC GAC CTA CA-3’ | 92.27 | 0.9926 |
Spz-R | 5′-TGT CGG TTT TGC AGA CGT AG-3’ |  |  |
CuZnSOD3-F | 5′-GGG AGA CCT AGG GAA CAT CC-3’ | 95.33 | 0.9920 |
CuZnSOD3-R | 5′-GTG GAT GAC CAC GGC TCT AT-3’ |  |  |
CASP-F | 5′-CTG CCC TGA ATT CCT CTC TG-3’ | 105.24 | 0.9885 |
CASP-R | 5′-CGA AGG TGG TAT GGA GCA AT-3’ |  |  |
Anv-F | 5′-AAT GGT GGT ATC AGC CTT GC-3’ | 94.53 | 0.9885 |
Anv-R | 5′-TTA GAG GGT CGA CCA TGA GG-3’ |  |  |
DICER-F | 5′-CAC TCG AGC ATC CTG TTT CA-3’ | 107.80 | 0.9969 |
DICER-R | 5′-ACC AAT CCC CAT CCA ATG TA-3’ |  |  |
HMCN1-F | 5′-TAA GGC AAC CGA CCA CTA CC-3’ | 107.85 | 0.9940 |
HMCN1-R | 5′-GAC GTA GAG ACT GGC GGA AG-3’ |  |  |
ARF-F | 5′-CCC ATT ACA GTG GTC CTG CT-3’ | 95.22 | 0.9928 |
ARF-R | 5′-CAG AAC CCT TCC CTT CAC AA-3’ |  |  |
ProPO-F | 5′-AAC AAC CTG AGA ACC GGA TG-3’ | 93.46 | 0.9899 |
ProPO-R | 5′-CGG CAG GGT TGG CAT AAT CT-3’ |  |  |
EF1a-F | 5′-TGC GCT GTG TTG ATT GTA GC-3’ | 103.19 | 0.9834 |
EF1a-R | 5′-ACA ATG AGC TGC TTG ACA CC-3’ |  |  |