Fig. 4

a. Experimental design of the selectivity test. All zygotes were injected with mCherry-Trim21 mRNA and OGDB, cultured to the 2-cell stage and injected in one blastomere either with water (b), with anti-GFP antibody (c), with anti-OCT4 antibody (d) or with anti-PDIA3 antibody (e). f. The same as (c), followed up to the blastocyst stage (day E3.5). In the right-hand column, mCherry fluorescence was quantified using Image-J. Size bar, 50 μm. OGDB, Oregon Green dextran beads. Statistical significance tested with Student’s t test. n.s., not significant. AU, arbitrary units of fluorescence intensity