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Fig. 3 | BMC Genomics

Fig. 3

From: Rapid adaptation of signaling networks in the fungal pathogen Magnaporthe oryzae

Fig. 3

[1] Stable reestablishment of osmoregulation in adapted strains. Vegetative growth of the Magnaporthe oryzae wildtype strain, mutants with inactivated components of the HOG signaling cascade and the “adapted” strains upon salt stress. The fungal colonies were grown on CM (upper row) and CM including 1 M KCl (lower row) for 7 d at 26 °C. A, B and C are representative for each plate. A = lof mutant, B = “adapted” strain taken directly from CM inclusive 1 M KCl (“adaptation” conditions), and C = “adapted” strain after six weeks of preincubation on CM (to have long-term unstressed conditions before this assay). In the case of the wildtype strain (left), the three cultures on each test plate used in the assays were from the M. oryzae wildtype strain 70–15 cultivated in an equal manner. [2] Mycelium dry weight of the Magnaporthe oryzae wildtype strain, mutants with inactivated components of the HOG signaling cascade and the “adapted” strains after growth in liquid culture upon KCl stress. The fungal colonies were grown in 250 ml CM including 1 M KCl for 6 d at 26 °C and 120 rpm. Error bars represent the standard deviation of three biological replicates of each strain

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