Fig. 1

Ribo-Zero RNA-Seq of chicken preadipocytes and adipocytes. a Procedure for inducing the differentiation of abdominal (Ab) and intramuscular (IM) preadipocytes (upper panel). Cells were collected for RNA-Seq at day 0 (Pre) and 10 days (Ad) (lower panel). Each stage included two biological replicates. Basal medium: DMEM/F12 + 10% FBS; differentiation induction medium: basal medium + DMI + oleate; maintenance medium: basal medium + insulin. The differentiation induction medium was replaced with maintenance medium after 2 days, and the maintenance medium was in turn replaced with basal medium at day four. b Oil Red O staining of preadipocytes (0d) and adipocytes (10d) in the IMF and AbF groups. Arrow indicates cytosolic lipid droplets. c qRT-PCR analysis of the adipogenic markers PPARG and FABP4 to confirm the identity of the chicken preadipocytes (mean ± SE, n = 3, **p ≤ 0.01). d Heatmap showing the results of correlation analyses between different samples. A correlation coefficient closer to 1 indicates a higher similarity between samples. e PCA of different samples based on the normalized expression levels of all expressed genes