Fig. 5

A comparison based on the F-score showed that TZMD slightly improved species differentiation. a Determining the TZMD cutoff with the highest F-score. b F-scores for 10 samplings with the TZMD method. c The highest F-scores for both TZMD and TETRA approaches. All 1779 queries against 264 references were used (Panel a). For each sampling, 200 intraspecific and 50,000 interspecific pairs were randomly sampled (Panels b and c). Because TETRA had two criteria (0.99 or 1.00) (Additional file 2: Figure S1A and S3A), the TETRA method using both criteria was compared. Dashed line, the TZMD cutoff at 0.21; P-value, one-tailed paired t-test