Skip to main content

Table 1 The effects of inclusion of a globin blocker in library construction on QuantSeq 3′ mRNA sequencing data in data set 1

From: The effects of a globin blocker on the resolution of 3’mRNA sequencing data in porcine blood

Biological replicate Globin blocker concentration Read counts by genes (millions) Number of expressed genes b
Total reads a Aligned reads Uniquely mapped reads Gene reads HBA reads HBB reads Non-globin reads
A 0 33.56 33.03 (98.41) 26.29 (78.32) 22.91 (68.25) 6.50 (19.38) 12.43 (37.02) 3.98 (11.85) 8437
  C1 36.23 35.44 (97.81) 22.31 (61.57) 13.96 (38.54) 2.76 (7.61) 0.92 (2.55) 10.28 (28.38) 9612
  C2 31.65 31.08 (98.20) 19.17 (60.57) 12.10 (38.21) 3.13 (9.87) 0.67 (2.10) 8.30 (26.24) 9692
  C3 27.05 26.48 (97.91) 16.83 (62.21) 11.44 (42.30) 4.26 (15.73) 0.96 (3.54) 6.23 (23.02) 9516
  C4 32.92 32.32 (98.17) 22.28 (67.66) 16.49 (50.08) 5.29 (16.07) 4.50 (13.67) 6.70 (20.34) 9270
B 0 34.12 33.44 (98.00) 25.19 (73.82) 23.68 (69.39) 8.77 (25.70) 11.54 (33.82) 3.37 (9.87) 7090
  C3 27.23 26.67 (97.92) 13.33 (48.96) 11.00 (40.38) 4.87 (17.88) 0.91 (3.34) 5.22 (19.15) 8547
  1. Abbreviations: HBA hemoglobin subunit alpha, HBB hemoglobin subunit beta
  2. The numbers in parentheses refer to percentages of the total reads (%)
  3. aThe number of reads after trimming by Bbduk
  4. bThreshold of expressed gene: ≥ 5 reads counts per 10 millions of total clean read counts