Table 1 The effects of inclusion of a globin blocker in library construction on QuantSeq 3′ mRNA sequencing data in data set 1
From: The effects of a globin blocker on the resolution of 3’mRNA sequencing data in porcine blood
Biological replicate | Globin blocker concentration | Read counts by genes (millions) | Number of expressed genes b | ||||||
|---|---|---|---|---|---|---|---|---|---|
Total reads a | Aligned reads | Uniquely mapped reads | Gene reads | HBA reads | HBB reads | Non-globin reads | |||
A | 0 | 33.56 | 33.03 (98.41) | 26.29 (78.32) | 22.91 (68.25) | 6.50 (19.38) | 12.43 (37.02) | 3.98 (11.85) | 8437 |
C1 | 36.23 | 35.44 (97.81) | 22.31 (61.57) | 13.96 (38.54) | 2.76 (7.61) | 0.92 (2.55) | 10.28 (28.38) | 9612 | |
C2 | 31.65 | 31.08 (98.20) | 19.17 (60.57) | 12.10 (38.21) | 3.13 (9.87) | 0.67 (2.10) | 8.30 (26.24) | 9692 | |
C3 | 27.05 | 26.48 (97.91) | 16.83 (62.21) | 11.44 (42.30) | 4.26 (15.73) | 0.96 (3.54) | 6.23 (23.02) | 9516 | |
C4 | 32.92 | 32.32 (98.17) | 22.28 (67.66) | 16.49 (50.08) | 5.29 (16.07) | 4.50 (13.67) | 6.70 (20.34) | 9270 | |
B | 0 | 34.12 | 33.44 (98.00) | 25.19 (73.82) | 23.68 (69.39) | 8.77 (25.70) | 11.54 (33.82) | 3.37 (9.87) | 7090 |
C3 | 27.23 | 26.67 (97.92) | 13.33 (48.96) | 11.00 (40.38) | 4.87 (17.88) | 0.91 (3.34) | 5.22 (19.15) | 8547 | |