Fig. 3
Nucleic acid sequence models of (a) region containing the coding exons split by a 1533 bp intron in the RJF leptin gene and (b) tcoding region within its mRNA. The start and stop codons, the dinucleotides at the RNA splicing site and the putative polyadenylation signal are shown in bold and indicated in blue, red and green respectively. Annealing sites for primers designed for PCR and RT-PCR are boxed. In (a), the protein sequence of the RJF leptin is shown in italics below the nucleic acid sequence of the coding regions. Complementary “CCCCCCCan” and “ttGGGGGGG” motifs are indicated in grey and yellow respectively. Single nucleotide polymorphisms with KT970642 sequence are indicated in red. In (b), motifs matching with the consensus of G4 structures (G3 + N1-12G3 + N1-12G3 + N1-12G3) are underlined and involved guanine stretches are shown in red