Fig. 4

Products obtained with PCR and RT-qPCR assays targeted on the chicken leptin gene. In (a), amplification products obtained using a PCR assay on the chicken leptin gene. Genomic DNA samples were purified from a blood samples of single females belonging to the RJF species (1), the alsacian old French chicken line (2), the Araucana chicken line (3), a white leghorn chicken line (4), the Gallus sonneratii species (5), the quail species Coturnix japonica (6), the turkey species Meleagris gallopavo (7), the pekin duck species Anas platyrhynchos domesticus (8) and the duck of barbary species Cairina moschata (9). Lane 10 shows a control sample without gDNA. Lane MW shows a 100-bp ladder with molecular sizes in base pairs indicated on the right. In (b), RT-qPCR results indicating the relative expression of the leptin gene