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Table 3 Comparison between the clustering expected for BP, MONO, and OTV probe types and the clustering produced by Affymetrix® pipelines based on the fluorescent intensity and contrast of 445 inbred lines for 479,027 probes

From: High throughput genotyping of structural variations in a complex plant genome using an original Affymetrix® axiom® array

  

Classification based on the clustering produced by Affymetrix® pipelines and genotyping assignment

Probe types

 

BP

OTV

    

BP

Number (%)

20,370 (43.9%)

26,012 (56.1%)

    

Clustering examples

    

Description

Two homoz. clusters

Two homoz. and one OT clusters

    
  

OTV

MONO

SNP

monomorphic

  

OTV

Number (%)

78,799 (81.3%)

502 (0.5%)

17,562 (18.1%)

4 (0.0%)

  

Clustering examples

  

Description

Two homoz. and one OT clusters

One homoz. and one OT clusters

Two homoz. clusters

One cluster

  
  

MONO

OTV

Unexpected MONO 1

SNP

Unexpected MONO 2

monomorphic

MONO

Number (%)

212,434 (63,3%)

15,690 (4,7%)

68,562 (20,4%)

1,981 (0.6%)

9,525 (2.8%)

27,586 (8.29%)

Clustering examples

Description

One homoz. and one OT clusters

Two homoz. and one OT clusters

One homoz., one OT and one het. clusters

Two homoz. clusters

One homoz. and one het. clusters

One cluster

  1. “Clustering example”: typical example of clustering based on the fluorescent intensity (y-axis) and contrast (x-axis). Colors on figure indicate the assignation of the genotype to the individuals based on this clustering; “Number (%)”: Number (percentage) of probes displaying the corresponding clustering. “Description”: Brief characteristic of each classification based on the clustering of individuals (homoz.= homozygote, het=heterozygous, OT= off-target)