Fig. 1

Determination of the cytotoxicity of zebularine and the chemicals effect on BmNPV proliferation. a BmN cells were treated with different concentrations of zebularine (20, 50, 100, 150, 200, 250 μM) for 12 h. Cytotoxicity was measured by MTT assay. Cellular cytotoxicity was determined in duplicate and each experiment was repeated three times. ** p < 0.01.b BmN cells were treated with 100 μM zebularine and infected with recombinant BmNPV BVs containing an egfp marker gene (Zeb/BmNPV). DMSO-treated BmN cells with recombinant BmNPV infection were used as the control (DMSO/BmNPV). Fluorescence intensity was observed under fluorescence microscopeat 72 hpi. c RNAs were extracted from BmNPV-infected BmN cells treated with zebularine and DMSO, respectively. Absolute qRT-PCR was carried out to analyze the copies of ie-1 and lef-3 at different time points. The data were represented as mean ± SD. Three independent experiments with three technical replicates were performed. d Western blotting analysis of VP39. Protein samples were from BmNPV-infected BmN cells with zebularine and DMSO, respectively. Western blot analysis for detection of VP39 was performed by an anti-VP39 antibody. a-Tubulin was served as the loading control