Fig. 5

Validation of both different expression and different methylation of BGIBMGA014008 in response to BmNPV infection. a BGIBMGA014008 was identified to be differentially expressed upon BmNPV infection by qRT-PCR. RNA was extracted from midguts with or without BmNPV infection. Data represent the relative transcript levels of genes in the infected midguts compared to the control tissues from three independent samples. The error bars indicate standard deviations (*p < 0.05). C group represents samples from normal midguts and T group representssamples from BmNPV-infected midguts. b The visualized image of differential methylation of BGIBMGA014008was generated by Integrative Genomics Viewer (IGV). The location of blue bars in the images indicates the methylated sites and the height of blue bars represents the methylation levels. c Bisulfite sequencing validation of differential methylation of BGIBMGA014008in normal verse infected midgut. Targeted bisulfite sequencing validation of a region overlapping the DMR is indicated by the rectangle over the genome browser tracks. Dark circles indicate methylated and open circles mean unmethylated cytosines. Each row consists of a single sequenced clone