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Fig. 3 | BMC Genomics

Fig. 3

From: The role of nitric oxide during embryonic wound healing

Fig. 3

Global gene expression profiles during embryonic wound healing. (a) Control embryos at stage 26 were injured using forceps and healing tissues were dissected (only the part marked by red rectangle) and collected for RNA-Seq analysis. (b-i) DEGs were grouped based on their expression profile relatively to 0 minutes and GO analysis was performed. (b, d, f, h) Expression profiles of genes are representative of the log transformed data, average gene expression is shown in red and expression of three representative genes are shown in green, purple and blue. (c, e, g, i) Genes, which have an annotation and human homolog, were used for GO analysis. Numbers of analysed genes are in the table together with the representative GO terms for each group. (j) Validation of RNA-Seq data by RT-qPCR using representative genes from each Group was performed using RT-qPCR and the Pearson r correlation coefficient was calculated from the geometric mean values. (RNA-Seq – three replicates, RT-qPCR – six replicates, geometric mean with geometric standard deviation). DEGs – differentially expressed genes, pw – post wounding

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