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Fig. 4 | BMC Genomics

Fig. 4

From: The role of nitric oxide during embryonic wound healing

Fig. 4

Changes in gene expression during wound healing after inhibition of NO production. (a) Graphical description of RNA-Seq experiment comparing control and NO inhibited embryonic wound healing. Only the part marked by red rectangle was collected and used for RNA isolation and sequencing. (b-g) DEGs, which were identified in RNA-Seq, were grouped based on their expression profile relatively to 0 minutes and GO analysis was performed. (b, d, f) Expression profiles of genes are representative of the z-score of the regularized log transformation of the normalized counts. (c, e, g) Genes with annotation and human homolog were used for GO analysis. Numbers of analysed genes are in the table together with the representative GO terms for each group. (h) RNA-Seq result of lep expression was verified (i) using RT-qPCR, separately for nos1-MO and nos3-MO. (j) Similarly, RNA-Seq result of fos expression was verified using (k) RT-qPCR (data are normalized to 0 minutes pw in controls, three replicates, geometric mean with geometric standard deviation, two-sided t-test from log2 values of relative expression between inhibited samples and control in 120 minutes pw), and (l) in situ hybridization. Site of injury is marked with a star and the signal where fos is expressed is circled by dot line (Scale bar = 100 μm) (M) Intensity of blue signal around site of injury were measured (one-way Anova, Dunnett’s multiple comparisons test, minimum 8 replicates). **** - p < .0001, ** - p < .01, * - p < .05, n.s. - p > .05 DEGs – differentially expressed genes, pw – post wounding, RIU – relative intensity unit

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