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Fig. 6 | BMC Genomics

Fig. 6

From: The role of nitric oxide during embryonic wound healing

Fig. 6

Monitoring of processes during wound healing after inhibition of lep expression. (a) In general, Lep is described as an activator of NO release, but inhibition of NO production leads to decreased expression of lep during wound healing. (b) Wound closing was documented using brightfield imaging on stereomicroscope. (c) Relative wound closure was calculated as the ratio between the size of the wound at 0 minute pw and 30 minutes pw (at least four replicates per condition, mean with standard deviation, the statistical difference between the groups is derived from two linear mixed models). (d) RT-qPCR comparison of temporal expression profiles of socs3 and (e) fos (data are normalized to 0 minutes pw in controls, three replicates, geometric mean with geometric standard deviation). (f) Actin at 30, 60 and 180 minutes pw visualized using green fluorescent phalloidin (Scale bar = 100 μm). (g) Laminin layer was visualized at 180 minutes pw and ends of the laminin layer are marked by a triangle (Scale bar = 100 μm). * - p < .05, n.s. – p > .05 pw – post wounding

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