Fig. 7

Validation of the expression patterns of genes induced in control and PvRbohB-RNAi roots. The expression levels of PvENOD40 (a), PvNIN (b), PvEF-HANDM (c), PvPO1 (d), PvCES17 (e), PvXGT (f), PvSAUR (g), PvERF1 (h), and PvACCO (i) were evaluated using RT-qPCR in P. vulgaris transgenic roots at 7 dpi with AM or rhizobia. The heatmaps of the expression of the corresponding genes, obtained using RNA-Seq, are shown below the graphs (a–i). The RT-qPCR data were normalized to the expression of PvEF1α. The RT-qPCR bars are the means ± SE of at least two independent biological replicates, with three technical repeats (n > 10). The asterisks represent statistically significant differences between the noninoculated and inoculated samples, as revealed using an unpaired Student’s t-test (*P < 0.05; **P < 0.01). The color bars of the heatmaps represent the Log2FoldChange, where red and blue represent the upregulated and downregulated genes, respectively