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Table 3 The results of qRT-PCR using ten randomly selected genes

From: PacBio single-molecule long-read sequencing shed new light on the complexity of the Carex breviculmis transcriptome

Gene annotation

ID

Tendency

Shade/Control (log2 FC)

Digital

qPCR

STAY-GREEN protein

F01.PB13572

Up

2.98

3.46

Chlorophyll a-b binding protein

F01.PB45249

Down

−2.44

−1.62

photosystem I reaction center subunit V

F01.PB53

Down

−1.72

−1.33

phospholipid hydroperoxide glutathione peroxidase 1

F01.PB15172

Up

2.14

1.76

root phototropism protein 2

F01.PB53223

Up

2.95

4.32

sucrose synthase 4

F01.PB11848

Up

3.84

2.41

WRKY transcription factor 70

F01.PB46640

Down

−4.38

−3.14

ethylene-responsive transcription factor ERF118

F01.PB53027

Up

1.11

1.61

scarecrow-like protein 21

F01.PB23994

Down

−2.15

−2.09

NAC domain-containing protein 100

F01.PB57159

No DEG

–

0.53