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Table 3 Bacterial strains used in this work

From: Genome rearrangements induce biofilm formation in Escherichia coli C – an old model organism with a new application in biofilm research

Strain Genotype Source
E. coli C WT Holly A. Wichman, James Bull, UofI, UT.
E. coli K12 MG1655 WT Lab collection
E. coli Crooks WT Lonnie Ingram, UF
E. coli B WT ATCC
E. coli W WT ATCC
E.coli K12 csrA csrA::mini-Tn5 KmR Tony Romeo, UF
E. coli K12 MG1655 EC100 FmcrA ∆(mrr-hsdRMS-mcrBC) φ80dlacZ∆M15 ∆lac
X74 recA1 endA1 araD139 ∆(ara, leu)7697 galU galK λ–rpsL nupG.
 pBBR1MCS-5 Broad host range mobilizable plasmid, GmR [54]
 pAG136 pET28B –EGFP-YFAST, KmR promoter probe vector [55]
 pProbe GFP [ASV] Short-life GFP promoter probe vector, KmR [56]
 pJEKd1750 1638-bp fragment with E.coli C IS3-csrA promoter in BglII/XbaI sites of pAG136, KmR This work
 pJEKd1751 1638-bp fragment with E. coli C IS3-csrA promoter in SmaI site of pProbe GFP [ASV], KmR This work
 pJEK718 csrA gene driven by the plac promoter in pBBR1MCS-5, GmR This work
 pJEK786 csrA gene driven by the pcat promoter in pBBR1MCS-5, GmR CmR This work
  1. Abbreviations: KmR, GmR, CmR – kanamycin, gentamycin, and chloramphenicol resistance