Table 3 Bacterial strains used in this work

E. coli C

WT

Holly A. Wichman, James Bull, UofI, UT.

E. coli K12 MG1655

WT

Lab collection

E. coli Crooks

WT

Lonnie Ingram, UF

E. coli B

WT

ATCC

E. coli W

WT

ATCC

E.coli K12 csrA

csrA::mini-Tn5 Km^R

Tony Romeo, UF

E. coli K12 MG1655 EC100

F⁻mcrA ∆(mrr-hsdRMS-mcrBC) φ80dlacZ∆M15 ∆lac

X74 recA1 endA1 araD139 ∆(ara, leu)7697 galU galK λ–rpsL nupG.

Lucigen

 pBBR1MCS-5

Broad host range mobilizable plasmid, Gm^R

[54]

 pAG136

pET28B –EGFP-YFAST, Km^R promoter probe vector

[55]

 pProbe GFP [ASV]

Short-life GFP promoter probe vector, Km^R

[56]

 pJEKd1750

1638-bp fragment with E.coli C IS3-csrA promoter in BglII/XbaI sites of pAG136, Km^R

This work

 pJEKd1751

1638-bp fragment with E. coli C IS3-csrA promoter in SmaI site of pProbe GFP [ASV], Km^R

This work

 pJEK718

csrA gene driven by the plac promoter in pBBR1MCS-5, Gm^R

This work

 pJEK786

csrA gene driven by the pcat promoter in pBBR1MCS-5, Gm^R Cm^R

This work