Table 1 Predicted gene targets for differentially expressed microRNAs (> 2 log2 fold, P < 0.0001) that correlate with S. carpocapsae strain behaviour. Differentially expressed S. carpocapsae microRNAs were assessed for binding sites across 5′ and 3’UTRs of all ion channel, innexin, GPCR, neurotransmitter and neuropeptide genes. Ce target refers to direct 1 to 1 gene orthologues that are biochemically confirmed microRNA targets in C. elegans [20]. U refers to the number of predicted interacting microRNAs following default microRNA target prediction; S refers to the number of predicted interacting microRNAs following strict microRNA target prediction. Within the implicated behaviour column, “Both (inverse int.)” refers to involvement in both behaviours, through polarised regulation states (significantly upregulated for one, and significantly downregulated for the other)
microRNA | Gene | Gene Name | Gene family | Ce target | D | S | Implicated behaviour | |
|---|---|---|---|---|---|---|---|---|
3’UTR | sc-miR-301-3p | L596_g12890.t2 | twk-12 | Ion Channel | y | 2 | 1 | Chemotaxis |
sc-miR-301-3p | L596_g12890.t3 | twk-12 | Ion Channel | Y | 2 | 1 | Chemotaxis | |
sc-miR-301-3p | L596_g12890.t1 | twk-12 | Ion Channel | y | 2 | 1 | Chemotaxis | |
sc-miR-759 | L596_g2695.t1 | acr-21 | Ion Channel | 2 | 1 | Nictation | ||
sc-miR-138 | L596_g3511.t1 | eat-5 | Innexin | 2 | 0 | Chemotaxis | ||
sc-miR-117 | L596_g24535.t1 | ador-1 | GPCR | 2 | 0 | Chemotaxis | ||
sc-miR-283 | L596_g24535.t1 | ador-1 | GPCR | 2 | 0 | Chemotaxis | ||
sc-miR-84-5pi | L596_g1430.t1 | ins-1b | Neuropeptide | y | 2 | 1 | Nictation | |
5’UTR | sc-miR-147 | L596_g23064.t1 | asic-2ii | Ion Channel | 2 | 2 | Chemotaxis | |
sc-miR-147 | L596_g14933.t1 | unc-36 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-156 | L596_g11170.t1 | egas-1 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-176 | L596_g11280.t1 | lgc-22 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-184 | L596_g11170.t1 | egas-1 | Ion Channel | 3 | 0 | Chemotaxis | ||
sc-miR-184 | L596_g13756.t1 | acr-25 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-190 | L596_g21438.t2 | unc-2 | Ion Channel | y | 3 | 3 | Chemotaxis | |
sc-miR-190 | L596_g21438.t1 | unc-2 | Ion Channel | y | 3 | 3 | Chemotaxis | |
sc-miR-190 | L596_g25880.t2 | slo-1 | Ion Channel | y | 2 | 2 | Chemotaxis | |
sc-miR-190 | L596_g25880.t1 | slo-1 | Ion Channel | y | 2 | 2 | Chemotaxis | |
sc-miR-211 | L596_g19116.t1 | unc-49 | Ion Channel | y | 5 | 5 | Chemotaxis | |
sc-miR-299 | L596_g11170.t1 | egas-1 | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-301-3p | L596_g11170.t1 | egas-1 | Ion Channel | 3 | 1 | Chemotaxis | ||
sc-miR-301-3p | L596_g23064.t1 | asic-2ii | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-301-5pi | L596_g9175.t1 | clhm-1 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-301-5pi | L596_g13756.t1 | acr-25 | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-301-5pii | L596_g13756.t1 | acr-25 | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-301-5pii | L596_g11170.t1 | egas-1 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-301-5pii | L596_g9175.t1 | clhm-1 | Ion Channel | 2 | 0 | Chemotaxis | ||
sc-miR-306 | L596_g21215.t1 | twk-46 | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-316 | L596_g14933.t1 | unc-36 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-316 | L596_g23064.t1 | asic-2ii | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-337 | L596_g9175.t1 | clhm-1 | Ion Channel | 2 | 0 | Chemotaxis | ||
sc-miR-338 | L596_g14875.t1 | kvs-5 | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-351 | L596_g15440.t1 | twk-43 | Ion Channel | 2 | 1 | Chemotaxis | ||
sc-miR-756 | L596_g9186.t2 | twk-2 | Ion Channel | 2 | 2 | Nictation | ||
sc-miR-756 | L596_g9186.t1 | twk-2 | Ion Channel | 2 | 2 | Nictation | ||
sc-miR-759 | L596_g15570.t1 | shk-1 | Ion Channel | y | 2 | 1 | Nictation | |
sc-miR-772 | L596_g15357.t1 | clh-5 | Ion Channel | 2 | 1 | Nictation | ||
sc-miR-772 | L596_g15357.t2 | clh-5 | Ion Channel | 2 | 1 | Nictation | ||
sc-miR-772 | L596_g11576.t1 | avr-14 | Ion Channel | y | 2 | 0 | Nictation | |
sc-miR-775 | L596_g20944.t1 | twk-29 | Ion Channel | 2 | 2 | Chemotaxis | ||
sc-miR-91 | L596_g12530.t1 | unc-58 | Ion Channel | 2 | 1 | Nictation | ||
sc-miR-759 | L596_g25584.t1 | unc-7 | Innexin | Y | 2 | 2 | Nictation | |
sc-miR-138 | L596_g9922.t1 | gar-2 | GPCR | y | 2 | 0 | Chemotaxis | |
sc-miR-151 | L596_g14843.t1 | npr-26 | GPCR | 2 | 2 | Both (inverse int.) | ||
sc-miR-184 | L596_g17321.t1 | npr-23 | GPCR | y | 2 | 0 | Chemotaxis | |
sc-miR-27 | L596_g22293.t1 | C30A5.10 | GPCR | 2 | 1 | Both (inverse int.) | ||
sc-miR-301-5pi | L596_g14261.t2 | npr-11 | GPCR | 2 | 1 | Chemotaxis | ||
sc-miR-351 | L596_g16439.t1 | srsx-24 | GPCR | 2 | 2 | Chemotaxis | ||
sc-miR-756 | L596_g17321.t1 | npr-23 | GPCR | y | 2 | 1 | Nictation | |
sc-miR-761 | L596_g25676.t1 | gar-1 | GPCR | 2 | 1 | Nictation | ||
sc-miR-773 | L596_g25676.t1 | gar-1 | GPCR | 2 | 0 | Nictation | ||
Sc-let-7 | L596_g13647.t1 | ins-1a | Neuropeptide | y | 2 | 1 | Nictation | |
sc-miR-301-3p | L596_g14298.t1 | nlp-49 | Neuropeptide | 2 | 1 | Chemotaxis | ||
sc-miR-84-5pi | L596_g5500.t2 | vglu-2 | Neurotransmission | 2 | 2 | Both (inverse int.) |