Fig. 2From: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolicsProduction of anthocyanins in leaves of N. benthamiana cv. NT following transient overexpression of Rubus Myb and bHLH regulatory genes in the presence or absence of a WDR component (TTG1). a Transient overexpression of flavonoid regulatory genes in N. benthamiana leaves at 3 days post infiltration (dpi) in comparison to the empty vector (ev) construct. The methanol extracts from each infiltration combination are presented below the infiltrated leaf used for extraction (i.e., 1.8-cm diameter leaf disc in 2 ml methanol: water: HCl (80:20:1, v/v/v). Bar = 1 cm. b Methanol extracts from N. benthamiana leaves (1.8-cm diameter leaf disc in 2 ml methanol: water: HCl (80:20:1, v/v/v) transiently expressing Rubus flavonoid regulatory genes with or without a WDR co-factor from 1 to 7 dpi. Extracts represent average absorbance values at 530 nm from eight leaf discs per time point. Leaf expression is shown at 7 dpi. Bar = 0.5 cmBack to article page