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Fig. 2 | BMC Genomics

Fig. 2

From: RNA-seq, de novo transcriptome assembly and flavonoid gene analysis in 13 wild and cultivated berry fruit species with high content of phenolics

Fig. 2

Production of anthocyanins in leaves of N. benthamiana cv. NT following transient overexpression of Rubus Myb and bHLH regulatory genes in the presence or absence of a WDR component (TTG1). a Transient overexpression of flavonoid regulatory genes in N. benthamiana leaves at 3 days post infiltration (dpi) in comparison to the empty vector (ev) construct. The methanol extracts from each infiltration combination are presented below the infiltrated leaf used for extraction (i.e., 1.8-cm diameter leaf disc in 2 ml methanol: water: HCl (80:20:1, v/v/v). Bar = 1 cm. b Methanol extracts from N. benthamiana leaves (1.8-cm diameter leaf disc in 2 ml methanol: water: HCl (80:20:1, v/v/v) transiently expressing Rubus flavonoid regulatory genes with or without a WDR co-factor from 1 to 7 dpi. Extracts represent average absorbance values at 530 nm from eight leaf discs per time point. Leaf expression is shown at 7 dpi. Bar = 0.5 cm

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