Table 1 Primers used in the study
Primer | Sequence (5′➔3′) | Assay |
|---|---|---|
16S rRNA F 16S rRNA R | ATG ACG GCC TTC GGG TTG TAA CGG CTG CTG GCA CGT AGT TG | RT-qPCR |
Rv0348 F Rv0348 R | AGC TCG CGG ACT ACG GCT TT GGT TCT CGC CGG TAA CTC CA | RT-qPCR |
devR F devR R | CCG ATC TGC GCT GTC TGA TC GTC CAG CGC CCA CAT CTT T | RT-qPCR |
mprA F mprA R | GAC CTG CCG ATT CTG GTG CT GGC GAA CGG CTT TGG TAG GT | RT-qPCR |
regX3 F regX3 R | TGA CGA CTA CGT GAC CAA GC CTC ATC TCC GAG TCG TCG TC | RT-qPCR |
lsr2 F lsr2 R | GGT CGA ATT CGG GCT TGA CG GCC ACC CAT TGC TTC AGG TC | RT-qPCR |
Rv0081 F Rv0081 R | CCT GGA GTC GTC GAA CCT GT GGG TGC GGC AAT CGA ATA GA | RT-qPCR |
Rv0678 F Rv0678 R | GCT GGC TGC TGG TGT GTG AT GGA TCA GCA TCC GGG CAT T | RT-qPCR |
mosRNcoI F mosRXhoI R | GCCGCCCCATGGCCATGACCATTTCGTTCTCTAGC GCCGCCCTCGAGCCGCTTGGGTCTTATCGAGATC | Cloning |
Rv3134c Up F Rv3134c Up R | GAC GGC CGC TGG TTC GGC AG TTA CTG GCG GGG ACC GCT ATC T | EMSA |