Fig. 1

Characterization and verification of differentially expressed genes. a Volcano plots obtained by using fold-change values and P-values; (b) Hierarchical clustering analysis of mRNAs that are differentially expressed between the female and male zebrafish samples. Each group contains three individuals and the expression values are represented in different colors; (c) Significantly differentially expressed genes by RT-qPCR. Gray and white bars represent the female and males, respectively. The error bars represent standard error (***, P < 0.001; and *, P = 0.05); and (d) Protein-protein interaction network. The green edges represent gene neighborhood, the red edges represent gene fusions, the blue edges represent gene co-occurrence, the black edges represent gene co-expression, and the yellow edges represent text mining