Fig. 3From: Structural and functional insights into the Diabrotica virgifera virgifera ATP-binding cassette transporter gene familySemi-quantitative PCR results of select D. v. virgifera ABC genes. a Developmental stage-specific expression profile of select transcripts. RNA isolated from Eggs (E), Larvae (L), Pupae (P), and adult Males (M) and Females (F) for each of the eight genes. DvvRPS6 was used as a positive control. b Assessment of target RNA levels in injected individuals. RNA was isolated 5 days after injection from pools of buffer injected (BI) individuals, and of dsRNA injected (KD) individuals. DvvRPS6 was used as a control to assess template qualityBack to article page