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Fig. 3 | BMC Genomics

Fig. 3

From: Genome-wide DNA methylation analysis of Metarhizium anisopliae during tick mimicked infection condition

Fig. 3

The impact of 5-Azacytidine treatment on methylated genes expression. Quantitative real time RT-PCRs of MANI_024437 (Destruxin synthetase); MANI_023437 (Xenolozoyenone-like polyketide synthase); MANI_111160 (Collagen-like protein Mcl1); MANI_026638 (Class 2 chitin synthase) and MANI_017257 (GPI-anchored cell wall beta-1,3-endoglucanase) were performed after growth of M. anisopliae E6 with R. microplus cuticles, as the sole carbon and nitrogen source, for 24, 48 and 72 h with and without 200 mM of 5-azacytidine (an DNMT inhibitor) supplementation. The results were processed according to 2-ΔCt method and relative transcript levels were normalized with beta-tubulin (MANI_018534). Data are shown as the mean ± SD from three experimental replicates of three biological replicates. * p < 0.05; ** p < 0.01

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