Fig. 4

The impact of 5-Azacytidine treatment on DNMTs expression. Quantitative real time RT-PCRs of MANI_011878 (DNA cytosine-5-methyltransferase) and MANI_017005 (RID1 DNA methyltransferase) were performed after growth of M. anisopliae E6 with R. microplus cuticles, as the sole carbon and nitrogen source, for 24, 48 and 72 h with and without 200 mM of 5-azacytidine (an DNMT inhibitor) supplementation. The results were processed according to 2^-ΔCt method and relative transcript levels were normalized with beta-tubulin (MANI_018534). Data are shown as the mean ± SD from three experimental replicates of three biological replicates