Table 3 Primers for construction and identification of FgGCN5 gene deletion mutant
Primer | Sequence |
|---|---|
280-1F | TAGCGTCTTCTCTTGATTGC |
280-1R | TTGACCTCCACTAGCTCCAGCCAAGCCATGATTGGTGCGGGCTCAAC |
280-2F | GAATAGAGTAGATGCCGACCGCGGGTTAACTAAAAGCGGGGAATCGG |
280-2R | ACCAAGACCTATCACAGCAC |
HYG-F | GGCTTGGCTGGAGCTAGTGGAGGTCAA |
HY-R | GTATTGACCGATTCCTTGCGGTCCGAA |
YG-F | GATGTAGGAGGGCGTGGATATGTCCT |
HYG-R | AACCCGCGGTCGGCATCTACTCTATTC |
JD280-F | TCGAAGAGCGCAATGGTG |
JD280-R | TAGCGAATCCGTGGCAAC |