Fig. 6

Alternative splicing in a WW domain containing protein gene (BART1_0-u51812). a. BART1_0-u51812 transcript models represented in the BaRTv1.0 database. b. AS events involving intron 2 validated by HR-RT-PCR. c. AS events between exon 6 and 8 validated by HR-RT-PCR. Electropherogram output from the ABI3730 shows the HR RT-PCR products (x-axis RT-PCR products (bp); y-axis relative fluorescence units). The products expected from RNA-seq are indicated as FS – Fully spliced, AE - Alternative exon, Alt 5’ss - Alternative 5′ splice site, IR-intron retention and Unspl.-Unspliced. * in B. indicates minor alternative transcripts identified in HR RT-PCR and in RNA-seq. + in C. indicates an uncharacterised alternative transcript identified in HR RT-PCR